SB (Sodium Borate) Buffer, 20x
Short protocol describing the production of 20x SB buffer for use in agarose gel electrophoresis.
45g Boric Acid
to 1 liter with MilliQ H20
Use this in your gel when making your agarose.
I normally use a 2% gel. 2% by weight of Agarose
to 1x SB buffer (assume it's the same density as
water, you really don't have to be that exact)
Also use 1x SB as your running buffer and SB in
your loading buffer. This is very important, otherwise
you will get smearing in your bands.
Lastly, the gel may be run at higher voltages, 300v or so
for no more than 5minutes for a 2% gel. If you use High
Melt agarose, you can probably run it longer. Also, if you
have a higher concentration gel you can run it longer. This
part I leave up to you to optimize.